Characteristics and distribution of large tandem duplications in brook stickleback (Culaea inconstans) mitochondrial DNA.
نویسندگان
چکیده
Most animal mitochondrial DNAs (mtDNAs) range in size from 15 to 18 kb, but increased sizes up to approximately 40 kb are occasionally found. We investigated large size variation in mtDNA of the brook stickleback fish, Culaea inconstans, and characterized four large (2.7-5.8 kb) tandem duplications. Duplications differ in size, frequency of occurrence, and degree of associated heteroplasmy, but each includes the control region and one or more adjacent genes. Duplications are correlated with two mtDNA lineages sampled from 31 populations. L1 duplications (3.2-4.8 kb) were present in all lineage I individuals (n = 121, 19 populations); 53 fish were heteroplasmic due to variation in the copy number of a tandemly repeated 270-bp sequence within the duplicated region. In contrast, duplications, L2, L3, and L4 (2.7-5.8 kb) occurred in only 117 of 174 lineage II fish, in eight of 14 populations. Nine fish with L3 or L4 duplications were heteroplasmic, possessing some mtDNAs that lacked duplications (normal-length mtDNAs). Heteroplasmy in L2 was associated with a small variable region near the ND5 gene. Phylogenetic analysis of restriction sites in Culaea mtDNAs and haplotype-defining sequence differences present in both copies argue for multiple independent events that gave rise to three of the four duplications.
منابع مشابه
Watershed characteristics shape the landscape genetics of brook stickleback (Culaea inconstans) in shallow prairie lakes
Investigating the consequences of landscape features on population genetic patterns is increasingly important to elucidate the ecological factors governing connectivity between populations and predicting the evolutionary consequences of landscapes. Small prairie lakes in Alberta, Canada, and the brook stickleback (Culaea inconstans) that inhabit them, provide a unique aquatic system whereby pop...
متن کاملDiversifying and correlational selection on behavior toward conspecific and heterospecific competitors in brook stickleback (Culaea inconstans)
Behaviors toward heterospecifics and conspecifics may be correlated because of shared mechanisms of expression in both social contexts (nonadaptive covariation) or because correlational selection favors adaptive covariation. We evaluated these hypotheses by comparing behavior toward conspecifics and heterospecifics in brook stickleback (Culaea inconstans) from three populations sympatric with a...
متن کاملTurnover of Sex Chromosomes in the Stickleback Fishes (Gasterosteidae)
Diverse sex-chromosome systems are found in vertebrates, particularly in teleost fishes, where different systems can be found in closely related species. Several mechanisms have been proposed for the rapid turnover of sex chromosomes, including the transposition of an existing sex-determination gene, the appearance of a new sex-determination gene on an autosome, and fusions between sex chromoso...
متن کاملPersistence of Dactylogyrus eucalius (Monogenea: Dactylogyridae) on the short-lived host Culaea inconstans (Pisces: Gasterosteiformes).
The monogene Dactylogyrus eucalius Mizelle and Regensberger, 1945 and its ability to maintain a population from year to year on the annual fish Culaea inconstans Kirkland was examined in a small lake in central Ontario. Fish were sampled toward the end of their annual breeding season, at a time when the host population consisted of 2 cohorts, i.e., young-of-the-year (0+) and mature adults (1+)....
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Genetics
دوره 145 2 شماره
صفحات -
تاریخ انتشار 1997